Inducible RFP Lentivirus

SC-030-I RFP inducible control lentivirus 200ul, ~1 x 10⁷ IFU/mL

Storage: <-70 °C, avoid repeat freeze/thaw cycles. Stable for 6 months at <-70oC.

1. Product Description:Lentivectors are HIV-1 (Human Immunodeficiency Virus 1) derived plasmids. They generate replication-incompetent lentivirus that can be transduced into almost all types of mammalian cells, including primary and non-dividing cells. Lentiviral particles (LP) are lentivirus supernatant generated from lentivectors express a specific gene or RNAi construction. Lentivirus is the easiest and most effective method for delivering genes into the majority of mammalian cell types, including non-dividing and primary cells. It allows genes to be integrated into the host cell genome for long-term expression.BioPioneer provides pre-made optional inducible expression lentiviruses for fluorescent proteins or specific human or mouse genes with various selection markers. To validate the inducible system in your cell lines, BioPioneer provides inducible lentivirus controls which have been generated from BioPioneer’s optional inducible lentiviral system. The inducible controls contain GFP or RFP under control of the optional inducible promoter, enabling convenient verification of tetracycline-inducible effects by simple monitoring of the GFP or RFP signal under a fluorescence microscope. The controls also contain a fluorescent-antibiotic fusion dual slection marker under a RSV promoter which is not affected by induction. The dual marker provides a convenient way to monitor viral transduction efficiency and select positive cells by either antibiotic killing or fluorescence sorting (See vector scheme above). The VSV-G pseudotyped lentiviral particles are generated in 293T cells, and are provided in two formats:

• DMEM containing 10% FBS
• PBS without any additives (suitable for serum sensitive culture or for the hard-to-transduce cells, or for in vivo applications.

SC-030-I (SC-030-I-Induceable-lenti_vrial_particles_RFP.doc, 153 kb)