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M-MuLv Reverse Transcriptase, 5000U
Recombinant reverse transcriptase, M-MuLv, 5000U.
Description
The M-MuLV Reverse Transcriptase (RT) is an RNA- and DNA-dependent DNA polymerase. It can use either RNA or DNA to prime DNA synthesis. The enzyme possesses a ribonuclease H activity specific to RNA in RNA-DNA hybrids
Source
E.coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase.
Molecular Weight
77.8 kDa monomer.
Applications
- Generation of first strand cDNA (3) for use in:
- PCR
- real-time PCR;
- second strand cDNA synthesis.
- DNA labeling
- Analysis of RNA by primer extension
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