2x Taq master mix with loading dye, 500 reactions

2x Taq master mix with blue loading dye, 500 reactions

The Taq Master mix contains recombinant Taq DNA polymerase and loading dye. It is for routine primer extension and efficient for amplification of template up to 5kb.

Content: It contains dNTPs, Taq DNA polymerase, Mg++, 2 x reaction buffer and loading dye. For each reaction, take 12.5ul to mix with your primer, template and make up to 25ul for the final volume with diH2O.

Each reaction contains 1U of Taq DNA polymerase, 1.5mM Mg++ and 400uM dNTPs.

Protocol:

Components / Volume (ul)

Supermix / 12.5 ul

Primers, Template, H20 / 12.5 ul

Template / X ul

Primer 1 / X ul

Primer 2 / X ul

H2O / X ul

Total / 25 ul