Mouse Tail Quick Extraction and Amplification Kit

MAQA-1 Quick extraction and amplification kit, 100 reactions

Storage:

1. Solution A, 10ml (Cat# MAQ-1)
2. Solution B, 10ml (Cat#MAQ-1)
3. Taq supermix with loading dye, 100 reactions (Cat# MAT-3.1)

1. Cut 0.2 cm tail snip from mice (or small section of tissue)

2. Place the tail snip into 100ul solution A in a 0.2ml PCR tube and incubate at 95⁰C using a PCR machine for 30-60 minutes.

3. Chill sample to room temperature and then vortex to mix the sample.

4. Add 100ul of solution B, vortex to mix

5. Use 1-2ul of above mixture to run PCR reaction immediately or store the mixture at -20^oC