RNase A, 250mg

RNase A, >60U/mg, 250mg

It cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide. The resulting 2', 3'-cyclic phosphate is hydrolyzed to the corresponding 3'-nucleoside phosphate.

Highlights

• RNase A is free of DNase activity. It is not necessary to heat it before use.

Applications

• Plasmid and genomic DNA preparation
• Removal of RNA from recombinant protein preparations

• Ribonuclease protection assays. Used in conjunction with RNase T1