hTMEM16A HEK293

Product Description
Recombinant HEK293 cell line expressing Shuman TMEM16A (transmembrane protein
16A, also called as anoctamin 1, calcium-activated chloride channel (ANO1), accession
number NM_018043).

Format
Each vial contains 1 X 10^6 cells in 1 ml of 10% DMSO.

Mycoplasma Testing
The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection
kit (Sigma-Aldrich) to confirm the absence of Mycoplasma species.

Introduction
Calcium-activated chloride channels (CaCCs) are major regulators in numerous
physiological processes including sensory transduction, epithelial secrection, cardiac and
neutonal excitation, and smooth muscle contraction.

TMEM16A (ANO1), a member of a family of putative plasma membrane proteins, is
identified as a CaCC that is activated by intracellular Ca2+ and Ca2+-mobilizing stimuli. It
has eight putative transmembrane segments without domains evidently involved in
calcium regulation. The relative permeability of TMEM16A to monovalent anions is
NO₃^- > I^- > Br^- > Cl^- > F^-.

References
Verkman A.S. and Galietta L.J.V. (2009) Cholide channels as drug targets. Nature
Reviews 8: 153-171

Caputo A. et al. (2008) TMEM16A, a membrane protein associated with calciumdependent
chloride channel activity. Science 322: 590-594

Yang Y.D. et al. (2008) TMEM16A confers receptor-activated calcium-dependent
chloride conductance. Nature 455: 1210-1215

Schroeder B.C. et al. (2008) Expression cloning of TMEM16A as a calcium-activated
chloride channel subunit. Cell 134: 1019-1029