BIO, 0.5 mg

BIO is a cell-permeable, potent, reversible and ATP-competitive GSK-3α/β inhibitor (IC₅₀ = 5 nM). Specificity was confirmed using various Cdk's (IC₅₀ = 83 nM for Cdk5/p25, 300 nM for Cdk2/cyclin A, 320 nM for Cdk1/cyclin B, and 10 µM for Cdk4/cyclin D1), MAP kinases, PKA, PKC isoforms, PKG, CK, and IRTK (IC₅₀ ≥ 10 µM). BIO is the first pharmacological agent shown to maintain self-renewal in human and mouse embryonic stem cells. Human embryonic stem cells (hESCs) are maintained in the undifferentiated state through treatment with a GSK-3 inhibitor, BIO, under a feeder-free condition.

CAS Number

667463-62-9

Chemical Formula

C₁₆H₁₀BrN₃O₂

Chemical Name

6-bromoindirubin-3′-oxime

Molecular Weight

356.17

QC Specifications

MASS Spec

Mouse ESC culture for toxicity

Shipping Information

on blue ice

Storage

4°C protected from light

Stability

Stable for up to 6 months from date of receipt when stored as directed.

Reference

1. Polychronopoulos, P., et al. 2004. J. Med. Chem. 47, 935.

2. Sato, N., et al. 2004. Nat. Med. 10, 55.

3. Meijer, L., et al. 2003. Chem. Biol. 10, 1255.