M-MuLv Reverse Transcriptase, 5000U

Recombinant reverse transcriptase, M-MuLv, 5000U.

Description
The M-MuLV Reverse Transcriptase (RT) is an RNA- and DNA-dependent DNA polymerase. It can use either RNA or DNA to prime DNA synthesis. The enzyme possesses a ribonuclease H activity specific to RNA in RNA-DNA hybrids

Source
E.coli cells with a cloned fragment of the pol gene encoding Moloney Murine Leukemia Virus reverse transcriptase.

Molecular Weight
77.8 kDa monomer.

Applications

• Generation of first strand cDNA (3) for use in:
  • PCR
  • real-time PCR;
  • second strand cDNA synthesis.
• DNA labeling
• Analysis of RNA by primer extension