FlashPrime High Fidelity DNA polymerase, 500U

Features

• High-speed, high fidelity DNA polymerase
• High processivity
• Fast amplification
• Shorter PCR runs for longer templates
• Robust, requires minimal optimization of the reaction

Applications

• Cloning techniques where high fidelity is desirable
• Blunt-end cloning
• Amplification of difficult templates
• Mutagenesis

DescriptionFlashPrime is a fast thermostable enzyme possessing 5´-3´ DNA polymerase and 3´-5´ proofreading exonuclease activities. FlashPrime provides high fidelity with an error-rate of 4.4 x 10^-7 combined with a high speed DNA synthesis as fast as 15s/Kb for templates of up to 5Kb and 30s/Kb for templates longer than 5Kb. The inherent high speed DNA synthesis is due to the enhanced processivity of the polymerase

FlashPrime is easy to use since it works with many different protocols and requires minimal optimization. The polymerase produces higher yields than most commercially available enzymes and generates blunt-ended amplicons. The error rate of FlashPrime DNA Polymerase (4.4 x 10^-7) was determined using the rpsL fidelity assay (Lackovich et al., 2001; Fujii et al., 1999).

Working ConcentrationOne unit will incorporate 10nmoles of dNTPs in 30min at 72°C.

Concentration2u/µl

Storage ConditionsFlashPrime DNA Polymerase can be stored for 12 months at –20°C

Shipping ConditionsOn Dry Ice or Blue Ice