Mon - Fri: 8:00am - 6:00pm

Pacific Time

1-(858) 689-6988

Phone

Endotoxin Removal Maxi Kit

Product Code: ER-003

Price: $220.00

- +
Endotoxin Removal Maxi Kit
1. Add 20ml of maxi plasmid into 20-mL Endotoxin removal Buffer in a 50-mL conical tube
2. Mix by inverting the tube a few times and put on ice for about 5-10 minutes until the solution is clear without turbidity;
3. Mix well again by inverting the tube a few times;
4. Incubate the tube at 37 ?C water bath for about 5-10 minutes and the solution shall be turbid or separated;
5. Centrifuge at 3,000 rpm in a low-speed centrifuge at room temperature for 5 minutes with the brake off;
6. Carefully transfer the upper clear layer solution to another 50-mL conical tube;
7. Precipitate plasmid DNA with 0.1 volume of 3 M NaAc (pH 5.4) and 2 volume of 100% ethanol.
Image

Contact Us

8540 Production Ave. Suit A,

San Diego 92121 USA

Phone: (858) 689-6988

Fax: (858) 225-0288

© Copyright 2024 Biopioneer. All Rights Reserved.